Saturday, November 30, 2019

Men will be Women Essays - Gender, Masculinity, Literature, Biology

Men will be Women Being men means something today that is completely different to the definition of past generations; This is true for any time period. Primitively, being a man meant to be fearless and tough in order to protect and feed their people, it was male nature. As time progresses, this definition was ever so slightly modified to a point where men are expected to be tamed and act as a male version of women. Men are animals by nature, and like animals men require certain necessities in order to keep calm. Chuck Palahniuk, in his book titled Fight Club, using toxic masculinity explores a fictional scenario in which men find a way to release this primitive tension and masculinity stored through the years from a macro-perspective in order to escape from a world where men are expected to suppress their male nature. Men are no longer the image of strong figures that put their lives in danger every day in order to provide and protect their people. From a macro-perspective, men have been changed alongside history itself in order to better fit social standards set by women. Fight Club explores toxic masculinity, the side of men that is not good for society. According to Harris O'Malley in his article titled, " The Difference Between Toxic Masculinity and Being A Man", Toxic masculinity is a concept with questionable origins that describes men who do not live up social standards, " for many people, the toxic ideas of masculinity are synonymous with being a man"(par 4). To simply be men is already a big offense. From a macro-perspective, men have been driven to avoid male nature by slowly turning men into what is believed to be the ideal man, a female version of men. From a micro-perspective, a male child told that he cannot cry because it is not the manly thing to d o, will store all the tension and eventually release it all at once. This is true for all the expectations for men today: boys will be boys, men think about sex every 7 seconds, men can't be friends with women, real men fight, etc. According to Kali Holloway , in her article titled "Toxic Masculinity Is Killing Men: The Roots of Men and Trauma", "...male infants actually behave in ways our society defines as feminine' " (par. 3), furthermore proving that we are both unconsciously and consciously driving men since childhood to be female. Kali Holloway states that "... social constructions of femininity demand that women be thin, beautiful, accommodating, and some unattainable balance of virginal and fuckable, social constructions of masculinity demand that men constantly prove and re-prove the very fact that they are, well, men" (par. 1), stating that men are not the only affected by social standards. Women are also affected, some may argue that even more than men. Holloway s tates, " B oth ideas are poisonous and potentially destructive, but statistically speaking, the number of addicted and afflicted men and their comparatively shorter lifespans proves masculinity is actually the more effective killer"(par 2). Though women may or may not be affected by more social standards, they are free to express their opinion and feelings on the matter. On the other hand, "...[men] are not only told they should suppress their emotions, but that their manliness essentially depends on them doing so" (par 7). Being a man means something completely different today, than it did the past generations. Being a man depends whether or not you can act according to female nature and the standards set by said nature. In Fight Club , the narrator finds the way to express his male nature through the way of Tyler, a persona created within himself. Tyler could do as he pleased and explore male nature at will, something the narrator has never been able to do. The narrator is the representation of men trapped in the standards of society that find a way to break free from such standards. Palahniuk describes the narrator as the way men should be according to social standards: white, heterosexual, with a degree, a job, and a furnished apartment. When the narrator realizes he checked all the boxed for the ideal man,

Tuesday, November 26, 2019

AF Environmental Threats Essays

AF Environmental Threats Essays AF Environmental Threats Essay AF Environmental Threats Essay What are the greatest environmental threats that have immediate implications for AF? Firebombed Fitch has been known for not being very diverse. They were striving for a certain look and style. It was mainly consisted of young white male and females with nice bodies, beautiful hair and shapes that fit their tiny clothes. If a customer were to walk into any AF store and look on the walls, they would notice that the models all have the same look. Not only do the models look the same, but was known for hiring associates that also fit that look. Firebombed has admitted to recruiting employees from sororities and fraternities and customers who looked the part. There were not many minorities working in the stores and even fewer minority models on the walls. If minorities were hired, they were working in the back, in stock. Or only coming in right before closing. Actions like these have opened the door for lawsuits for discrimination. Racial profiling, has also limited the type of clientele that frequents the stores. While this was exactly what AF was owing for Initially, It Limits the potential Income that the company could be earning. By not being as diverse as many other clothing companies, AF could stand the chance of losing business. Many customers do not care for discrimination and the misrepresentation of cultures. Firebombed and Fitch have four brands such as Hollister, Ruler, Firebombed and Giggly Hicks. A negative mark on one brand can possibly negatively affect all brands. 2. What are the greatest opportunities available in the marketplace for AF to pursue? In light of the economy decreasing due to the housing market taking a dive and the unemployment rate rising everyday, people are not as willing to spend a large amount of money on clothes. Now is the time for many to become conservative, save money and shop for bargains. To maintain their customers and possibly gain more, AF could take advantage of the bargain 2. What are the greatest opportunities available In the marketplace for AF to pursue? AF could take advantage of the bargain Identify and describe the greatest environmental threats that have immediate implications for AF. Firebombed Fitch has been known for its lack of diversity. They have always strives for a particular look and style. Their staff mainly consists of young white attractive male and females. Store Managers are encouraged to ask attractive shoppers If they want to apply for a sales position. AF has outlined a strict dress policy that the employees hair have to be neat and attractive, make up Is to be worn to en hance natural features along with many other demands. In the stores there are posters of attractive went models wearing trademark clotting wanly represents ten look they wanted to capture when hiring employees. AF recruits employees from sororities and fraternities who look the part and fit their mold of the all American male and female. AF ran into legal issues when lawsuits were filed alleging that they discriminated against minorities. It was said that in an effort to cultivate a white workforce minority applicants were steered into less visible Jobs such as stock

Friday, November 22, 2019

Cell Biology and Biotechniques

The review of the literature by Assadi et al. (2016) highlights how biophysical technology has been efficiently utilized in addressing research questions and finding effective results in clinical investigation. This research study mainly utilized the method of flow cytometry and transfection technique apart from real time PCR and immunoflourescence microscopy to detect fatty acid metabolism-immune nexus (FAMIN) in Chrohn’s disease risk gene LACC1 (Laccase domain containing 1) encoded protein in human tissues. This method of fatty acid metabolism was decided because genetic variation in the LACC1 gene increased the risk of Chrohn’s disease. The main of this research was to elucidate the biological function of LACC1 gene and determine the characterization of the gene expression in human tissue and cells. The research question for the study was how characteristics expression of LACC1 gene can increase risk of Crohn’s disease (Assadi et al. 2016). The FAMIN protein encoded by the LACC1 gene control all the defense mechanism of human body and the research was important to gain additional insights into how FAMIN characterization and LACC1 gene expression may create risk of Crohn’s disease. The transfection technique was used by maintaining human cell line THP-1 in suitable medium and the cells were incubated wit phorbol-12-myristate 13-acetate (PMA) to induce macrophage differentiation. For transfection experiment the HeLa cell line was transfected with empty vectors and LACC1 vector constructs. Western blotting was done on this cell and expression level was calculated. As transfection technique lead to unexpected morphologies and abnormalities in target cells, it helped in studying the expression of gene.   On the other hand, immunofluoroscence staining was used to calculate the number of peroxisomes and several antibodies were used both for immunofluorescence staining and western blot (Assadi et al. 2016).Hence, trans fection technique was applied in this research to study macrophage differentiation and gene expression of LACC1 gene. A research which considers the efficiency and cytotoxic effect of tranfection methodology lead to better results, whereas highly cytotoxic approaches lead to unwanted effects and unknown changes in gene expression which may affect the results (Goehring et al. 2014).   Flow cytometry is the biophysical technology done by means of laser and used to analyze the characteristics of particle in a fluid stream by passing through electronic detection device. .   Flow cytometry technique is widely used in the cell tracking, cell viability and infection studies and to conduct functional analysis in cell signaling gene expression studies (Givan 2013). In the research study by Assadi et al. (2016), flow cytometry of human blood derived cell and granulocytes and erythrocytes were isolated from the cell and stained using monoclonal antibodies. The FAMIN cells labeled with antibodies and data analyzed with flow cytometer and flow analysis software. This technique helped in the characterization of FAMIN expression in THP-1 cells. The result indicated the FAMIN expression was not detected in untreated cells, however PMA induced differentiation was seen during THP-1 (positive) cell lines. Hence the result of the study finally showed that LACC1 gene codes for macr ophage immune-metabolic function and polymorphic variation influences risk of Crohn’s disease (Assadi et al. 2016). Considering the clinical use of flow cytometry in research studies, the main advantage of flow cytometry is its ability to characterize immunophenotyping deviation in cells and detect diagnosis of hematopoietic disorders like leukemia. It has led to new methods for assessing hematologic malignancy and MRD. It is cost-effective and quick method for disease detection and diagnosis. However the limitation of the method is that it cannot easily detect sarcoma and carcinomas as they tend to stick (Woo et al. 2014). The main principle of fluorescence and fluorescence microscopic techniques was used to analyze therapeutic effectiveness in Crohn’s disease. In patients with Crohn’s disease, the antibody to tumor necrosis factor (TNF) binds to the membrane bound TNF and suppresses immune response in person. Hence, the researcher used fluorescent antibodies for TNF imaging studies. Despite using TNF therapy, patient show little improvement in their clinical symptoms and it also in creases the risk of side-effects like allergic reaction and skin disorders. The main limitation in treatment was that clinician cannot predict the response to anti-TNF therapy in patients. With this problem in treatment of patients with Crohn’s disease, the research question for the study was to see how fluorescence antibodies to TNF can help in studying response to anti-TNF therapy (Atreya et al. 2014).   Therefore, the use of different techniques like transfection, fluorescence microscopy and flow cytometry helped in studying variation in expression of Crohn’s disease risk gene and increased understanding of Crohn’s disease risk gene. Higher LACC1 expression was seen in spleen and lymph nodes in this research study. Hence application of this technique helped in gaining better understanding of LACC1 function in regulating immune-mediated function and diseases. These tools can be exploited in the future for several diagnostic studies. Assadi, G., Vesterlund, L., Bonfiglio, F., Mazzurana, L., Cordeddu, L., Schepis, D., Mjà ¶sberg, J., Ruhrmann, S., Fabbri, A., Vukojevic, V. and Percipalle, P., 2016. Functional Analyses of the Crohn’s Disease Risk Gene LACC1.  PloS one,  11(12), p.e0168276. Atreya, R., Neumann, H., Neufert, C., Waldner, M.J., Billmeier, U., Zopf, Y., Willma, M., App, C., Mà ¼nster, T., Kessler, H. and Maas, S., 2014. In vivo imaging using fluorescent antibodies to tumor necrosis factor predicts therapeutic response in Crohn's disease.  Nature medicine,  20(3), pp.313-318. Givan, A.L., 2013.  Flow cytometry: first principles. John Wiley & Sons. Goehring, A., Lee, C.H., Wang, K.H., Michel, J.C., Claxton, D.P., Baconguis, I., Althoff, T., Fischer, S., Garcia, K.C. and Gouaux, E., 2014. Screening and large-scale expression of membrane proteins in mammalian cells for structural studies.  Nature protocols,  9(11), pp.2574-2585. Woo, J., Baumann, A. and Arguello, V., 2014. Recent advancements of flow cytometry: new applications in hematology and oncology.  Expert review of molecular diagnostics,  14(1), pp.67-81.

Wednesday, November 20, 2019

PRoject Research Paper Example | Topics and Well Written Essays - 2000 words

PRoject - Research Paper Example Its main components are feldspar and quartz, even though, there are small amounts of amphiboles, mica, and numerous other elements. Due to the varying composition of the granite rock, it comes in various shades and colors such as white, pink, red and gray. Mable, on the other hand, is the metamorphic rock that will be formed when limestone is exposed to the pressure and heat of the metamorphism. Its main component is calcium calcite and normally can have other minerals such as quartz, clay minerals, graphite, iron oxide, pyrite, and micas. Under the intense condition of metamorphosis, the calcite components of the limestone rejoin each other and form larger calcite crystals. Nigeria has its unique culture that is also in cooperated into their business world. One is likely to find self spending in the first two hours of a first conversation just exchanging pleasantries and talking about matters of family and health. To them, these matters are of great importance. One might find himself trying to rush things at this stage. Something that is worth noting is that Nigerians often work as a team and closed door meetings are bound to be frequently interrupted by phone calls, emails, and errands. Some scholars have argued that the law of supply and demand is enough to shape the business world in any region, however, others argue that politics is very vital for business in any region of the world. However what is now known to be true is that lots of political factors determine which direction the economy swings. The ruling government in Nigeria often introduces new rules and regulations or eliminate old ones that determine how business transactions in the country are to be countries out. By this, may include; waivers, import promotion decrees, tariffs, import duties and industrial promotion policies. It can is best illustrated by the 2004 import waiver of Dangote Industries that enabled the firm to

Tuesday, November 19, 2019

Choose at least 4 classic political theorists to discuss in depth. Are Essay

Choose at least 4 classic political theorists to discuss in depth. Are their theories still influential today - Essay Example Aristotle’s views on politics were based upon the politician as the main player in a political system. In this sense, the biggest role for the politician is the creation and putting in place of laws. The laws collectively make up the constitution that governs the people of any given state. The constitution contains the laws, institutions, and customs of the people and shall determine the courses of action that shall be taken in the cases of conflict. The politician’s role after the constitution is in place is to maintain it, introduce reforms when necessary, and to prevent any developments meant to subvert the political system in general. Aristotle calls this the province of legislative science and holds it in higher regard than politics as it is exercised in the normal sense with the passing of mere decrees. Aristotle’s political theory is very influential and still has influence on today’s political landscape. It is evident that today’s politician s craft laws besides defending the course of the constitution. Any efforts that may subvert the political system in place are usually dealt with by the politician through bills and debates which involve voting just as Aristotle theorized. Plato was Aristotles teacher and their political theories are similar in many ways. His major political work was â€Å"The Republic† and it contained his ideas on an effective and efficient political system. His political system was majorly concerned with justice and defined every state in terms of its levels of justice. An efficient Platonian political system comprised of a merchant class that dealt with the economic structure, a military class that handled security and a political structure in the hands of philosopher-kings. People’s classes should be determined by the educational process in place and available to the specific individual. Those who would have completed the educational process in whole would become philosopher kings and they

Saturday, November 16, 2019

Commercial Law Flow Charts and Notes Essay Example for Free

Commercial Law Flow Charts and Notes Essay You must take reasonable care to avoid acts or omissions which you can reasonably foresee would be likely to injure your neighbour- Who, then, in law, is my neighbour? The answer seems to be persons who are so closely and directly affected by my act that I ought reasonably to have them in contemplation as being so affected when I am directing my mind to the acts or omissions that are called in question Donoghue v Stevenson Neighbour Principle: You must take reasonable care to avoid acts or omissions which you can reasonably foresee would be likely to injure your neighbour- Who, then, in law, is my neighbour? The answer seems to be persons who are so closely and directly affected by my act that I ought reasonably to have them in contemplation as being so affected when I am directing my mind to the acts or omissions that are called in question Donoghue v Stevenson Reasonable Person Test individual action or failure to act as a reasonably prudent person would under similar circumstances, resulting in harm to another Blyth v Birmingham Waterworks Co (1856) Papatonakis v Australian Telecommunications Commission (1985) That it is appropriate for the negligent person’s liability to extend to the harm so caused This was stated in Section 5D of the Civil Liability Act 2002 (NSW) and is consistent with the case of Adeels Palace Pty Ltd v Moubarak Other Tests: for a causal link to exist these elements must be satisfied: iii. Negligence was a necessary condition for the occurrence of the harm iv. That it is appropriate for the negligent person’s liability to extend to the harm so caused This was stated in Section 5D of the Civil Liability Act 2002 (NSW) and is consistent with the case of Adeels Palace.

Thursday, November 14, 2019

Mary Shelleys Frankenstein and John Miltons Paradise Lost Essay

Mary Shelley's Frankenstein and John Milton's Paradise Lost â€Å"Forth reaching to the Fruit, She pluck’d, she eat:/ Earth felt the wound, and Nature from her seat/ Sighing through all her Works gave signs of woe,/ That all was lost [†¦]† (PL 8. 781-784) In the gothic novel Frankenstein, Mary Shelley weaves an intricate web of allusions through her characters’ expedient desires for knowledge. Both the actions of Frankenstein, as well as his monster allude to John Milton’s Paradise Lost. Book eight of Milton’s story relates the tale of Satan’s temptation and Eve’s fateful hunger for knowledge. The infamous Fall of Adam and Eve introduced the knowledge of good and evil into a previously pristine world. With one swift motion sin was birthed, and the perfection of the earth was swept away, leaving pain and malevolence in its wake. The troubles of Victor Frankenstein begin with his quest for knowledge, and end where all end: death. The characters in Frankenstein are a conglomeration of those in Paradise Lost. Frankenstein parallels Adam and Eve in the Garden of Eden, as well as God, while his monster acts an Eve/Satan mixture. The most predominant theme of this novel is the characters’ ever-present search for knowledge. It is this thirst for learning that spurs Frankenstein’s psychotic attempts to give life to inanimate tissue, ultimately causing his demise. Frankenstein, in this way, mirrors the character of Eve in Paradise Lost. Eve lives her most peaceful life in the Garden of Eden, her only job being to tend the plants in the Garden which she loves so much. In the novel Frankenstein, Frankenstein lives in an Eden of his own, though macabre in nature. His â€Å"garden of life† is actually mo... ...was influenced greatly by Milton’s work, evidence of which lies in the eerie similarities between the two. The allusions to Paradise Lost give the reader a story by which to subconsciously compare the characters of Frankenstein, thus also reiterating one of the main themes; the quest for knowledge and the resultant death. Following the death of Frankenstein, his monster utters his own last words. â€Å"‘But soon,’ he cried, [†¦] ‘I shall die. [†¦] I shall ascend my funeral pyre triumphantly, and exult in the agony of torturing flames’† (225). Works Cited Milton, John. Paradise Lost. 1667. Electronic Text Center, University of Virginia Library. 20 Nov. 2005. id=MilPL67.sgm&images=images/modeng&data=/texts/english/ modeng/parsed&tag=public&part=8&division=div1> Shelley, Mary. Frankenstein. 1818. New York: Penguin Classics, 2003.